• Nair et al 1996:
    • Few patients had pain; most had extensive caries or coronal breakdown.
    • All teeth were extracted with the lesion attached.
    • Represented an “unknown fraction” of all extracted teeth with radiolucencies.
    • Utilized complete serial sectioning and microscopy (Light and TEM).
  • Abbott 1999:
    • Biopsies taken during surgery.
    • Most teeth had undergone Root Canal Re-treatment before surgery.
    • Half had previous apical surgery, especially with retrograde amalgam.
    • Many had over-extended previous RCFs or continual pain/infection despite re-treatment.
    • Utilized light microscopy on only a few sections.
  • Love & Firth 2009, Schultz et al 2009, Wang et al 2004, Becconsall-Ryan et al 2010:
    • Biopsies taken during surgery for radiolucencies persisting >4 years or enlarging.
    • Some patients had pain.
    • RCT was not possible or had already been performed with no healing.
    • Utilized light microscopy on up to four sections.